Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Soluble Terminal Complement Complex C5b-9 (sC5b-9). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Soluble Terminal Complement Complex C5b-9 (sC5b-9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Soluble Terminal Complement Complex C5b-9 (sC5b-9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Soluble Terminal Complement Complex C5b-9 (sC5b-9) in the samples is then determined by comparing the OD of the samples to the standard curve